Col Rabih Alenezi says he was ordered to evict villagers from a tribe in the Gulf state to make way for The Line, part of the Neom eco-project.
One of them was subsequently shot and killed for protesting against eviction.
The Saudi government and Neom management refused to comment.
Neom, Saudi Arabia’s $500bn (£399bn) eco-region, is part of its Saudi Vision 2030 strategy which aims to diversify the kingdom’s economy away from oil.
Its flagship project, The Line, has been pitched as a car-free city, just 200m (656ft) wide and 170km (106 miles) long - though only 2.4km of the project is reportedly expected to be completed by 2030.
At least Saudi Arabia is doing something to guard against the imminent collapse of their oil economy… So that’s good, I guess.
Not sure Neom is the project I would have funded to do so, but I imagine the Saudis are getting desperate.