Landscape with Charon Crossing the Styx is an oil on wood painting by the Flemish Northern Renaissance artist Joachim Patinir. Dating to c. 1515–1524, it is now in the Museo del Prado, in Madrid.
Landscape with Charon Crossing Styx fits into common Northern Renaissance and early Mannerist trends of art. The 16th century witnessed a new era for painting in Germany and the Netherlands that combined influences from local traditions and foreign influences. Many artists, including Patinir, traveled to Italy to study and these travels provided new ideas, particularly concerning representations of the natural world. Patinir’s religious subjects, therefore, incorporate precise observation and naturalism with fantastic landscapes inspired by the northern traditions of Bosch.
https://en.wikipedia.org/wiki/Landscape_with_Charon_Crossing_the_Styx
I don’t think that they did.